| Functions |
Requirements |
| Network of closed loop channels |
- Rectangular shape
- Polydimethylsiloxane (PDMS)
- diameter of channels: 100nm – 900μm
|
| Multiple in vitro model application |
- PDMS allows for numerous biological applications:
- CTCs
- Hematologic Diseases
- Polymerase Chain Reactions
|
- Chemical signal isolation
|
- Plasmolyzed PDMS to prevent signal molecule absorption
|
| Device platform does not degrade chemical signaling molecules |
- PDMS is organic biocompatible
- it is non-toxic
- low chemical reactivity
|
| Endothelialization of the channels |
- Monolayer of endothelial cells can adhere to device channels
- Fibronectin concentration of 2µg/cm2
|
| Imageable for long term studies |
- PDMS is transparent
- Use of small heating elements and element placement do not obstruct view of channels
|
| Thermal convection |
- Use of integrated Nichrome wire to heat the device, and create temperature gradient
- Create the buoyancy flow
- PDMS thermal conductivity (0.15 W/m K)
|
| Controlling Temperature Gradient |
- Thermocouple
- Controller
- Temperature accuracy of ± 0.5 °C
|
| Device should be no bigger than 1inch x 3 inch in total |
- Device should fit on standard 1 x 3 inch glass slide
- Has to fit in imaging device
|
| Flow velocity |
|
